![]() ![]() Copy gate to group no longer overwrites modified downstream gates.Exporting offset histogram overlays to pdf no longer pushes the histogram off the graph.Boolean gates dragged within a sample no longer reference the original population.Hiding rows in the compensation matrix no longer causes editing offset issues.Additionally, HTTPS for authentication is checked on by default. When checked this will reveal a check box that allows the user to have FlowJo remember the FlowJo Portal credentials they used to sign in for 24 hours. Improved FlowJo Portal Sign In: There is now a ‘Show Remember Me’ check box in the license preferences.A handful of XML reading adjustments were made to translate group membership better, align time parameter reading, and handle multiple files with the same name. Version 10.8 will be the best version of 10 to directly open. Jo file reading: FlowJo version 9.x produces.This will allow for better interoperability between BD FACSDiva TM and FlowJo users. ![]() However, as we work toward better alignment between all software in our control, we have chosen to align the two compensation methods and normalize to the largest number. Importantly, neither of these approaches are wrong fluorescence values are relative, and all measures would remain relatively the same, so there is no need to re-analyze spectrally compensated data. Spectral compensation in BD FACSDiva TM instead normalizes to the largest number. FlowJo continued to normalize to the ‘primary’ detector and allowed for larger numbers. In spectral compensation, where there is no longer a 1:1 relationship between number of detectors and collected parameters, the largest fluorescence value may not be in the primary detector. For traditional compensation approaches this will always be the largest value and the resulting diagonal will be 100% of the signal for the given fluorochrome. Spectral compensation: FlowJo to this point has normalized all compensation matrices to the ‘diagonal’, meaning the value in the primary detector.Flow Jo should pick the positive & negative populations automatically. To use the spectral compensation feature, you’ll need FlowJo version 10.6 or later (FCS express now has this feature also)Ĭlick the compensation icon and select the parameters you are using. If signals are brighter in non-primary detectors we have observed non-optimal spreading occurring. Do not delete them!Ĭheck if signals in their primary detectors are the brightest. Pairing beads with beads with the same lot number, cells with cells)įluorochromes used for compensation must be the same as the sample (Can’t compensate AF647 with APC for example) The positive and negative populations must have the same auto-fluorescence (i.e. Positive controls have to be equally bright or brighter than the sampleĪll signals are within the linear detection range You’ll record single stained controls just like conventional compensation. It can be done on any cytometer as long as you collect n+1 data points where n is the number of colours in your cytometry panel. The more light (data) you collect, the better the unmixing can perform. Spectral compensation utilises signals from non-primary detectors. The additional information allows the software to separate individual colours more precisely, decreasing spillover. ![]()
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